Genetic variability of human coronavirus OC43‐, 229E‐, and NL63‐like strains and their association with lower respiratory tract infections of hospitalized infants and immunocompromised patients
Identifieur interne : 004273 ( Main/Exploration ); précédent : 004272; suivant : 004274Genetic variability of human coronavirus OC43‐, 229E‐, and NL63‐like strains and their association with lower respiratory tract infections of hospitalized infants and immunocompromised patients
Auteurs : Giuseppe Gerna [Italie] ; Giulia Campanini [Italie] ; Francesca Rovida [Italie] ; Elena Percivalle [Italie] ; Antonella Sarasini [Italie] ; Antonietta Marchi [Italie] ; Fausto Baldanti [Italie]Source :
- Journal of Medical Virology [ 0146-6615 ] ; 2006-07.
English descriptors
- Teeft :
- Aspirate, Assay, Bronchiolitis, Cell culture, Cell cultures, Cell line, Cell lines, Clin, Coinfections, Consecutive seasons, Coronavirus, Coronavirus infection, Coronavirus pneumonia, Coronaviruses, Different hcovs, Freymuth, Genetic variability, Gerna, Hcov, Hcov coinfections, Hcov infection, Hcov infections, Hcov strains, Hcovs, Hku1, Hrsv, Hrsv hcov, Human coronavirus, Human coronavirus infections, Human metapneumovirus, Immunocompetent, Immunocompetent patients, Immunocompromised, Immunocompromised patients, Infection, Mcintosh, Monoclonal, Monoclonal antibodies, Nasopharyngeal, Nasopharyngeal aspirates, Other hand, Pancov, Patient population, Percivalle, Phylogenetic, Phylogenetic analysis, Pneumonia, Present study, Primer, Primer pairs, Proc natl acad, Reference strain, Reference strains, Respiratory disease, Respiratory secretions, Respiratory syndrome, Respiratory tract disease, Respiratory tract infections, Respiratory viruses, Rhinovirus, Sarasini, Single infections, Syncytial virus, Syndrome, Total number, Tract infection, Tract infections, Untypeable, Untypeable hcov strains, Untypeable strains, Vabret, Viral, Virol, Young children.
Abstract
In the winter–spring seasons 2003–2004 and 2004–2005, 47 (5.7%) patients with acute respiratory infection associated with human coronavirus (hCoV) 229E‐, NL63‐, and OC43‐like strains were identified among 823 (597 immunocompetent and 226 immunocompromised) patients admitted to hospital with acute respiratory syndromes. Viral infections were diagnosed by either immunological (monoclonal antibodies) or molecular (RT‐PCR) methods. Each of two sets of primer pairs developed for detection of all CoVs (panCoV) failed to detect 15 of the 53 (28.3%) hCoV strains identified. On the other hand, all hCoV strains could be detected by using type‐specific primers targeting genes 1ab and N. The HuH‐7 cell line was found to be susceptible to isolation and identification of OC43‐ and 229E‐like strains. Overall, hCoV infection was caused by OC43‐like, 229E‐like, and NL63‐like strains in 25 (53.2%), 10 (21.3%), and 9 (19.1%) patients, respectively. In addition, three patients (6.4%) were infected by untypeable hCoV strains. NL63‐like strains were not found to circulate in 2003–2004, and 229E‐like strains did not circulate in 2004–2005, while OC43‐like strains were detected in both seasons. The monthly distribution reached a peak during January through March. Lower predominated over upper respiratory tract infections in each age group. In addition, hCoV infections interested only immunocompetent infants and young children during the first year of life, while all adults were immunocompromised patients. Coinfections of hCoVs and other respiratory viruses (mostly interesting the first year of life) were observed in 14 of the 47 (29.8%) patients and were associated with severe respiratory syndromes more frequently than hCoV single infections (P = 0.002). In conclusion, the use of multiple primer sets targeting different genes is recommended for diagnosis of all types of hCoV infection. In addition, the detection of still untypeable hCoV strains suggests that the number of hCoVs involved in human pathology might further increase. Finally, hCoVs should be screened routinely for in both infants and immunocompromised patients with acute respiratory infection. J. Med. Virol. 78:938–949, 2006. © 2006 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jmv.20645
Affiliations:
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<front><div type="abstract" xml:lang="en">In the winter–spring seasons 2003–2004 and 2004–2005, 47 (5.7%) patients with acute respiratory infection associated with human coronavirus (hCoV) 229E‐, NL63‐, and OC43‐like strains were identified among 823 (597 immunocompetent and 226 immunocompromised) patients admitted to hospital with acute respiratory syndromes. Viral infections were diagnosed by either immunological (monoclonal antibodies) or molecular (RT‐PCR) methods. Each of two sets of primer pairs developed for detection of all CoVs (panCoV) failed to detect 15 of the 53 (28.3%) hCoV strains identified. On the other hand, all hCoV strains could be detected by using type‐specific primers targeting genes 1ab and N. The HuH‐7 cell line was found to be susceptible to isolation and identification of OC43‐ and 229E‐like strains. Overall, hCoV infection was caused by OC43‐like, 229E‐like, and NL63‐like strains in 25 (53.2%), 10 (21.3%), and 9 (19.1%) patients, respectively. In addition, three patients (6.4%) were infected by untypeable hCoV strains. NL63‐like strains were not found to circulate in 2003–2004, and 229E‐like strains did not circulate in 2004–2005, while OC43‐like strains were detected in both seasons. The monthly distribution reached a peak during January through March. Lower predominated over upper respiratory tract infections in each age group. In addition, hCoV infections interested only immunocompetent infants and young children during the first year of life, while all adults were immunocompromised patients. Coinfections of hCoVs and other respiratory viruses (mostly interesting the first year of life) were observed in 14 of the 47 (29.8%) patients and were associated with severe respiratory syndromes more frequently than hCoV single infections (P = 0.002). In conclusion, the use of multiple primer sets targeting different genes is recommended for diagnosis of all types of hCoV infection. In addition, the detection of still untypeable hCoV strains suggests that the number of hCoVs involved in human pathology might further increase. Finally, hCoVs should be screened routinely for in both infants and immunocompromised patients with acute respiratory infection. J. Med. Virol. 78:938–949, 2006. © 2006 Wiley‐Liss, Inc.</div>
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